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MORIKAWA, Keiko MORIKAWA, Keiko KASHINO, Genro KASHINO, Genro MORI, Hiromu MORI, Hiromu gemcitabine radiosensitization CHO xrs5 DNA DSBs gemcitabine radiosensitization CHO xrs5 DNA DSBs application/pdf Abstract : Gemcitabine (4-amino-1- [3,3- difluoro -4- hydroxyl -5- (hydro-xymethyl) Tetrahydrofuran -2- yl] - 1H – pyrimidin -2- one; dFdC) is a deoxycytidine analogue which is well-known for its anti-tumor activity especially in pancreatic cancer. It is one of the more effective drugs to sensitize cells for radiation and its radio-sensitizing properties were demonstrated both in vivo and in vitro. Yet, the exact interaction of dFdC and radiation has not been elucidated. In this study, we examined the mechanisms by using 10 MV Lineac-X-irradiation on culture mammalian cells. A repair deficient clone, xrs5 cell line was used in addition to wild type rodent cell line (CHO). The sensitivity to each irradiation or dFdC on cells was evaluated by colony formation assay, and much higher cell killing effects were observed. To learn about the mechanism more, we examined p-53-binding protein 1 (53BP1) focus formation by the immunofluorescent method, which is known as a method for the detection of DNA double-strand breaks (DSBs). The results showed that the treatment of dFdC alone could induce 53BP1 foci, suggesting that DNA DSBs are induced by dFdC independently. When cells were irradiated after the dFdC treatment, enhanced cell-killing effects were observed in CHO cells but not in xrs5. These results strongly suggest that dFdC inhibit the repair for DNA DSBs. 純真学園大学 2014-03 jpn departmental bulletin paper VoR https://junshin.repo.nii.ac.jp/records/91 40020087030 AA12581584 2186-6481 純真学園大学雑誌 Journal of Junshin Gakuen University, Faculty of Health Sciences 3 053 059 https://junshin.repo.nii.ac.jp/record/91/files/純真学園大学雑誌-03号_p053-059.pdf application/pdf 992.0 kB 2016-10-11