{"created":"2023-05-15T12:13:41.347082+00:00","id":126,"links":{},"metadata":{"_buckets":{"deposit":"5cd9105e-53fd-4500-9688-6d5aac9d7627"},"_deposit":{"created_by":12,"id":"126","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"126"},"status":"published"},"_oai":{"id":"oai:junshin.repo.nii.ac.jp:00000126","sets":["1:6:88:13"]},"author_link":["185","221","220","184","190","222","188","186","187"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"95","bibliographicPageStart":"89","bibliographic_titles":[{"bibliographic_title":"純真学園大学雑誌"},{"bibliographic_title":"Journal of Junshin Gakuen University, Faculty of Health Sciences","bibliographic_titleLang":"en"}]}]},"item_10002_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"要旨：白血病は., 血液中の血球が腫瘍化する血液の癌であり., 急性白血病と慢性白血病に大別される. 現在では化学療法や造血幹細胞移植により完治することが可能となりつつあるが., 他の固形癌と同様に化学療法は患者への負担が非常に大きい治療法となっている. そこで我々は., 抗がん剤について検討するに当たりChrysophyllum cainito（現地呼称：ミルクフルーツ）の種子が強い細胞溶解作用を持つことを以前に行った実験より見出し., この種子からの抽出液は白血病細胞株を死滅させることを明らかにした. また., この作用がどのような機序で起こるのかを知るためにChrysophyllum cainito種子による細胞死がアポトーシスにより引き起こされているかを検討した. 急性前骨髄球性白血病株であるHL60と急性単球性白血病細胞株であるU937をアポトーシスマーカーであるAnnexin Vと生細胞には取り込まれず死細胞のみを染色するPropidium Iodide（PI）で染色し., その蛍光強度をFlow cytometerを用いて比較検討した. その結果., 細胞はアポトーシスにより細胞死を引き起こした一方で., ネクローシスによる細胞死は起きていないことが示唆された. また., この作用にはHL60とU937細胞株において作用が異なることからミルクフルーツ種子抽出物は細胞種毎に作用が異なっており., これらのことは., ミルクフルーツ種子抽出物が細胞死を誘導する際に細胞の選択を行っている可能性も示唆される結果となった. \n\nAbstract : Leukemia, blood cell change to tumorigenic hematological cells that is divided into acute leukemia and chronic leukemia. It is possible that leukemia is cured using chemotherapy, hematopoietic stem cell transplantation, etc. Concurrently, the patient burdens was increased by chemotherapy for tumors include leukemia and other solid cancer. We revealed that the seed of Chrysophyllum cainito (Common name: Milk fruit) has a strong hemolytic action and extracts from the seeds induce cell death for leukemic cell line.　Here, we measured fluorescent intensity in order to examine whether seeds of C. cainito induce apoptosis. Fluorescent intensity were compared by FITC conjugated AnnexinV for late apoptotic maker and Propidium Iodide for count of death cells using a flow cytometry in a human acute promylocytic leukemia cell line, HL60 and human acute monocytic lukemia cell line, U937. As a result, the cells were induced cell death by apoptosis. Meanwhile, we could not observed necrosis in each cell line. The result shown that the effect by seeds of C. cainito are different in each cell line. Therefore it become the result that extract of C. cainito seeds select against cell type in inducing cell death.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"純真学園大学"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"40020905169","subitem_relation_type_select":"NAID"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12581584","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2186-6481","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日下, 雅友"},{"creatorName":"クサカ, サマトモ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSAKA, Masatomo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"186","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677405","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677405"}]},{"creatorNames":[{"creatorName":"一原, 直人"},{"creatorName":"イチハラ, ナオト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"220","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677406 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677406 "}]},{"creatorNames":[{"creatorName":"秋山, 秀彦"},{"creatorName":"アキヤマ, ヒデヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"185","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677404","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677404"}]},{"creatorNames":[{"creatorName":"天川, 雅夫"},{"creatorName":"アマカワ, マサオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"221","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677403","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677403"}]},{"creatorNames":[{"creatorName":"Pros, Try"},{"creatorName":"Try,  Pros","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"188","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677407","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677407"}]},{"creatorNames":[{"creatorName":"加藤, 亮二"},{"creatorName":"カトウ, リョウジ","creatorNameLang":"ja-Kana"},{"creatorName":"KATO, Ryoji ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"190","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000000233833 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000000233833 "}]},{"creatorNames":[{"creatorName":"ICHIHARA, Naoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"187","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677406","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677406"}]},{"creatorNames":[{"creatorName":"AKIYAMA, Hidehiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"222","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000330677404 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000330677404 "}]},{"creatorNames":[{"creatorName":"AMAKAWA, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"184","nameIdentifierScheme":"WEKO"},{"nameIdentifier":" 9000330677403","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/ 9000330677403"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-07-15"}],"displaytype":"detail","filename":"純真学園大学雑誌-05号_p089-095.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"純真学園大学雑誌-05号_p089-095","url":"https://junshin.repo.nii.ac.jp/record/126/files/純真学園大学雑誌-05号_p089-095.pdf"},"version_id":"dd3ea686-58c1-4fc8-b7c5-21d16030a907"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アポトーシス","subitem_subject_scheme":"Other"},{"subitem_subject":"天然成分","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞溶解作用","subitem_subject_scheme":"Other"},{"subitem_subject":"白血病","subitem_subject_scheme":"Other"},{"subitem_subject":"血液成分","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Natural ingredient","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Lytic","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Leukemia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"blood component","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト血液細胞株に対する細胞特異的なミルクフルーツ種子抽出物の細胞死誘導についての検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト血液細胞株に対する細胞特異的なミルクフルーツ種子抽出物の細胞死誘導についての検討"},{"subitem_title":"Effect of cell-specific cell death induction using extract of Chrysophyllum cainito seeds against human hematological cell line include hematological tumor","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["13"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-07-15"},"publish_date":"2017-07-15","publish_status":"0","recid":"126","relation_version_is_last":true,"title":["ヒト血液細胞株に対する細胞特異的なミルクフルーツ種子抽出物の細胞死誘導についての検討"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-05-15T15:41:14.646589+00:00"}