{"created":"2023-05-15T12:13:43.426618+00:00","id":171,"links":{},"metadata":{"_buckets":{"deposit":"2784c14b-274f-43a3-bb24-2f7d859d1c12"},"_deposit":{"created_by":12,"id":"171","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"171"},"status":"published"},"_oai":{"id":"oai:junshin.repo.nii.ac.jp:00000171","sets":["1:7:17:36"]},"author_link":["276"],"control_number":"171","item_10002_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"内分泌かく乱化学物質Bisphenol-Aと17β-estradiolによる臍帯静脈内皮細胞(HUVECs)Endothelin合成系の下方制御","subitem_alternative_title_language":"ja"}]},"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"48","bibliographicPageEnd":"156","bibliographicPageStart":"145","bibliographic_titles":[{"bibliographic_title":"純真紀要"},{"bibliographic_title":"Bulletin of Junshin Junior College","bibliographic_titleLang":"en"}]}]},"item_10002_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【Abstract】\n Endothelin is a potent vasoconstrictor. Our goal was to investigate the role of the endocrine disruptor bisphenol A (BPA), and 17 β-estradiol (E2) in the regulation of endothelin-1 (ET-1) mRNA expression and ET-1 release. \n Methods: Cultured human umbilical vein endothelial cells (HUVECs) were incubated ± BPA (0.01-100 μ mol/L) and E₂ (0.001-10 μ mol/L)  for 48h. We determined cell viability (measured using a cell counting kit), ET-1 release (measured by EIA) and preproendothelin-1 ( ppET-1) mRNA levels (evaluated by RT-PCR) after 0h, 24h and 48h.\nResults: Cell viabillity decreased to 89% (p<0.05) with 10 μ mol/L E₂ and to 78% (p<0.05)  with 100 μ mol/L BPA.Endothelin release and ppET-1 mRNA were measured using lower, non-toxic E₂ and BPA doses. ET-1 release decreased to 87% and 84% with 10 and 100 nmol/L E₂ and to72% and 65% with 1 and 10 μ mol/L BPA (p<0.05 for all). E₂ (100 nmol/L) decreased ppET-1 mRNA leaves to 75% (p<0.05) and 85% after 24h and 48h, respectively. BPA (10μmol/L) decreased ppET-1 mRNA levels to 61% (p<0.05) and 80% after 24h and 48h, respectively.\nConclusion: E₂ and BPA directly attenuate ET-1 gene expression and decrease ET-1 release. E₂ will play the essential role in the regulation of vascular tome besides the function as a sex hormone.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"【要旨】\nEndothelim（ET-1)は、血管内皮細胞から産出される協力な血管収縮ペプチドである。我々は、内分泌かく乱化学物質のBispheno-A（BPA)と内因性の17β-Estradiol(E₂）が血管内皮細胞のET-1産生（ET-1放出とpreproET-1mRNA発現）に対しどのような影響を与えるのか検討した。【方法】培養ヒト臍帯静脈内皮細胞（HUVECs）を48時間、BPA（0.01-100μmol/L)とE₂（0.001-10μmol/L)に暴露した。0時間後、24時間後、48時間後の細胞生残率（cell counting kitを用い測定）、ET-1放出（EIA法にて測定）とpreproET-1（ppET-1）mRNA発現（RT-PCRにて評価）を測定した。【結果】細胞残生率は、非暴露対照と比べ、10μmol/LのE₂で89%（p<0.05)まで、そして、100μmol/LのBPAで78%（p<0.05）まで減少した。ET-1放出は、非暴露対照と比べ、10μnmol/L、100nmol/LのE₂でそれぞれ87%、84%まで、1umol/L、10μmol/LのBPAでそれぞれ72%、65%まで減少した（すべてp<0.05）。ppET-1mRNA発現は、非暴露対照と比べ、E₂（100nmol/L）では24時間、48時間後にそれぞれ75%（p<0.05）、85%まで、BPA(10μmol/L）では24時間、48時間後にそれぞれ61%(p＜0.05)、80%まで低下した。【考察】E₂が直接ET-1遺伝子発現を低下させ、血液内皮細胞からのET-1放出を減少さることが明らかになった。このことは、E₂は性ホルモンとして機能の他に血管緊張の調節で重要な役割を演じていることを示唆するものである。また、内分泌かく乱化学物質であるBPAがE₂同様にppET-1mRNA発現を送静し、ET-1放出を減少させることも明らかにした。しかし、この研究で用いたBPA濃度は他の研究者が血漿中で得たものより遥かに高濃度であったので、内分泌かく乱化学物質のBPAが生体において血管緊張に影響を及ぼすことはないだろうと推定した。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"純真短期大学","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"40016100949","subitem_relation_type_select":"NAID"}}]},"item_10002_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"純真短期大学","subitem_rights_language":"ja"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0011314X","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855651","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1710308327429":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Down-regulation of The Endothelin System by The Endocrine Disruptor Bisphenol-A, and 17 β-estradiol in Human Umbilical Vein Endothelial Cells（HUVECs）","subitem_title_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000 0004 0639 7440","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000406397440"}],"affiliationNames":[{"affiliationName":"純真短期大学","affiliationNameLang":"ja"},{"affiliationName":"Junshin Junior College","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"KUNIFUJI, Yasumasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"276","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000010550547","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000010550547"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bisphenol-A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"endocrine disruptors","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"endothelin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human umbilical vein endothelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"17β-estradiol","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Down-regulation of The Endothelin System by The Endocrine Disruptor Bisphenol-A, and 17 β-estradiol in Human Umbilical Vein Endothelial Cells（HUVECs）","item_type_id":"10002","owner":"12","path":["36"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-11-10"},"publish_date":"2017-11-10","publish_status":"0","recid":"171","relation_version_is_last":true,"title":["Down-regulation of The Endothelin System by The Endocrine Disruptor Bisphenol-A, and 17 β-estradiol in Human Umbilical Vein Endothelial Cells（HUVECs）"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-06-11T08:31:38.840942+00:00"}