{"created":"2024-06-24T09:58:05.038903+00:00","id":2000052,"links":{},"metadata":{"_buckets":{"deposit":"6bd7aa30-5ebe-456a-83dc-f44e63c90c27"},"_deposit":{"created_by":12,"id":"2000052","owner":"12","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2000052"},"status":"published"},"_oai":{"id":"oai:junshin.repo.nii.ac.jp:02000052","sets":[]},"author_link":[],"control_number":"2000052","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-31"},"bibliographicIssueNumber":"14","bibliographicPageEnd":"76","bibliographicPageStart":"69"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The detailed pathway by which gemcitabine-induced DNA double-strand breaks (DSBs) are repaired has not been elucidated. We evaluated the possible role of gemcitabine by fluorescent immunostaining on a rodent cell line. A repair-deficient clone, the xrs5 cell line, was used in addition to a wild-type rodent cell line (CHO). We observed changes in the number and size of CHO and xrs5 cells after 24 h of treatment with gemcitabine at a concentration of 5.0 μM. The results showed that the cellular lethality exerted by gemcitabine involves cell enlargement, and the number of DNA DSBs induced by gemcitabine was significantly higher in xrs5 cells than in CHO cells. In terms of changes in DNA DSBs over time, CHO cells showed a decreasing trend, while xrs5 cells showed an increasing one. The findings confirmed that gemcitabine inhibits the repair of radiation-induced DSBs via a pathway other than non-homologous end joining.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_full_name_3":{"attribute_name":"著者（英）","attribute_value_mlt":[{"names":[{"name":"MORIKAWA, Keiko","nameLang":"en"}]},{"names":[{"name":"YOSHIDA, Yukito","nameLang":"en"}]},{"names":[{"name":"SUGII, Yuh","nameLang":"en"}]},{"names":[{"name":"KASHINO, Genro","nameLang":"en"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"純真学園大学","subitem_publisher_language":"ja"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12581584","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2186-6481","subitem_source_identifier_type":"ISSN"}]},"item_1710308327429":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"【原著】Effect of gemcitabine on mammalian cells by fluorescent immunostaining: examination of cell enlargement and DNA double-strand breaks","subitem_title_language":"en"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森川, 惠子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"9000414239392","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000414239392"}]},{"creatorNames":[{"creatorName":"吉田, 幸人","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"杉井, 裕","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"菓子野, 元郎","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-26"}],"displaytype":"detail","filename":"原著_Effect of gemcitabine on mammalian cells by fluorescent immunostaining_examination of cell enlargement and DNA double-strand breaks.pdf","filesize":[{"value":"6.5 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"url":"https://junshin.repo.nii.ac.jp/record/2000052/files/原著_Effect of gemcitabine on mammalian cells by fluorescent immunostaining_examination of cell enlargement and DNA double-strand breaks.pdf"},"version_id":"1e39a0ed-1a9f-44c7-a337-05fd4e894620"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"gemcitabine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CHO","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"xrs5","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA double-strand break","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell enlargement","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"【原著】Effect of gemcitabine on mammalian cells by fluorescent immunostaining: examination of cell enlargement and DNA double-strand breaks","item_type_id":"10002","owner":"12","path":["1714035455460"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-26"},"publish_date":"2024-06-26","publish_status":"0","recid":"2000052","relation_version_is_last":true,"title":["【原著】Effect of gemcitabine on mammalian cells by fluorescent immunostaining: examination of cell enlargement and DNA double-strand breaks"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-06-26T09:42:37.932136+00:00"}