@article{oai:junshin.repo.nii.ac.jp:00000593,
 author = {矢野, 博之},
 issue = {13},
 journal = {純真学園大学雑誌, Journal of Junshin Gakuen University},
 month = {Mar},
 note = {Radiation-induced fibrosis (RIF) is thought to be the result of the excessive accumulation of collagen and other extracellular matrix components. Previously, we reported that the TGF-β/Smad signaling pathway plays an important role in the expression of type I collagen during RIF. Type V collagen is incorporated into fibers expressing type I collagen and has an important role in controlling the diameter of collagen fibers. It is thought that type V collagen plays a major role in the radiation-induced increase in type I collagen expression, but its role in the development of RIF remains unclear. microRNAs (miRNAs) are small, noncoding sequences approximately 20 nucleotides long that negatively regulate the gene expression at the post-transcriptional level. We previously reported that miR-29 negatively regulates the expression of type I collagen in RIF. However, the effect of miR-29 on regulating the expression of type V collagen remains unclear. In the present study, we cultured mouse fibroblasts (NIH-3T3 cells) and examined the regulation of the Col5a1 expression at the transcriptional and post-transcriptional level by miRNAs using real-time PCR and Luciferase assay. The expression of type V collagen were increased by irradiation at the transcriptional level. Ionizing radiation increased the 3ʹ untranslated region activity of Col5a1 while decreasing the expression of miR-29. This indicates that ionizing radiation cancelled the negative regulation of Col5a1 by miR-29. In conclusion, we showed that the expression of Col5a1 was increased by irradiation and that this increment was coordinated at the transcriptional and miR-29-mediated post-transcriptional levels.},
 pages = {83--90},
 title = {【原著】Regulation of the pro-α1(V) collagen gene (Col5a1) following ionizing radiation at the transcriptional and post-transcriptional levels.},
 year = {2023},
 yomi = {ヤノ, ヒロユキ}
}