{"created":"2023-05-15T12:14:04.176551+00:00","id":598,"links":{},"metadata":{"_buckets":{"deposit":"0f0b3575-e640-4a8f-ac53-5069120581da"},"_deposit":{"created_by":12,"id":"598","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"598"},"status":"published"},"_oai":{"id":"oai:junshin.repo.nii.ac.jp:00000598","sets":["1:6:82:99"]},"author_link":["1006"],"control_number":"598","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageEnd":"128","bibliographicPageStart":"121","bibliographic_titles":[{"bibliographic_title":"純真学園大学雑誌"},{"bibliographic_title":"Journal of Junshin Gakuen University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Gemcitabine (dFdC) is a deoxycytidine analogue that is well known for its anti-tumor activity, especially in pancreatic cancer. It is an effective drug for sensitizing cells to radiation, but the exact interaction of dFdC and radiation has not been elucidated. In this study, we evaluated the effect of gemcitabine on radiosensitization in mammalian cells using immunofluorescence (IF) to examine DNA double-strand breaks (DSBs) induced in cell nuclei. To detect the site of DNA DSBs, IF staining was performed to observe phosphorylated p53-binding protein 1 (53BP1)-positive foci over time. Cells were treated with or without 5 μM gemcitabine for 24 hours prior to treatment with or without post-γ-ray irradiation. Gemcitabine could induce DNA DSBs without irradiation, which were not repaired over time. After irradiation, significantly higher numbers of 53BP1 foci were observed in cells pre-treated with gemcitabine than in untreated cells. Higher numbers of 53BP1 foci were confirmed to remain over time in the cells treated with both gemcitabine and irradiation. Overall, our data suggest that the enhanced cell killing effect observed with gemcitabine treatment is dependent on the inhibition of cellular DNA DSB repair mechanisms.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"純真学園大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12581584","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2186-6481","subitem_source_identifier_type":"ISSN"}]},"item_1710308327429":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"【資料】Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells","subitem_title_language":"en"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森川, 惠子"},{"creatorName":"モリカワ, ケイコ","creatorNameLang":"ja-Kana"},{"creatorName":"MORIKAWA, Keiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1006","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000414239392","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000414239392"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-19"}],"displaytype":"detail","filename":"Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells.pdf","filesize":[{"value":"7.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells","url":"https://junshin.repo.nii.ac.jp/record/598/files/Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells.pdf"},"version_id":"9f099034-f1be-47dc-afd3-7cc47c09e5eb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Radiosensitizationgemcitabine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gemcitabine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"53BP1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA double-strand break","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immunofluorescence","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"【資料】Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells","item_type_id":"10002","owner":"12","path":["99"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-19"},"publish_date":"2023-04-19","publish_status":"0","recid":"598","relation_version_is_last":true,"title":["【資料】Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2025-03-06T08:25:15.648102+00:00"}