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The effect of radiosensitization by gemcitabine on mammalian cells
https://junshin.repo.nii.ac.jp/records/91
https://junshin.repo.nii.ac.jp/records/919c903fd2-dd62-4ebc-98ee-a16668e640e0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
純真学園大学雑誌-03号_p053-059 (992.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-10-11 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gemcitabine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | radiosensitization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CHO | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | xrs5 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DSBs | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | gemcitabine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | radiosensitization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | CHO | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | xrs5 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DSBs | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
MORIKAWA, Keiko
× MORIKAWA, Keiko× KASHINO, Genro× MORI, Hiromu× MORI, Hiromu |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Abstract : Gemcitabine (4-amino-1- [3,3- difluoro -4- hydroxyl -5- (hydro-xymethyl) Tetrahydrofuran -2- yl] - 1H – pyrimidin -2- one; dFdC) is a deoxycytidine analogue which is well-known for its anti-tumor activity especially in pancreatic cancer. It is one of the more effective drugs to sensitize cells for radiation and its radio-sensitizing properties were demonstrated both in vivo and in vitro. Yet, the exact interaction of dFdC and radiation has not been elucidated. In this study, we examined the mechanisms by using 10 MV Lineac-X-irradiation on culture mammalian cells. A repair deficient clone, xrs5 cell line was used in addition to wild type rodent cell line (CHO). The sensitivity to each irradiation or dFdC on cells was evaluated by colony formation assay, and much higher cell killing effects were observed. To learn about the mechanism more, we examined p-53-binding protein 1 (53BP1) focus formation by the immunofluorescent method, which is known as a method for the detection of DNA double-strand breaks (DSBs). The results showed that the treatment of dFdC alone could induce 53BP1 foci, suggesting that DNA DSBs are induced by dFdC independently. When cells were irradiated after the dFdC treatment, enhanced cell-killing effects were observed in CHO cells but not in xrs5. These results strongly suggest that dFdC inhibit the repair for DNA DSBs. | |||||
| 書誌情報 |
純真学園大学雑誌 en : Journal of Junshin Gakuen University, Faculty of Health Sciences 号 3, p. 053-059, 発行日 2014-03 |
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| 出版者 | ||||||
| 出版者 | 純真学園大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2186-6481 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12581584 | |||||
| 論文ID（NAID） | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | NAID | |||||
| 関連識別子 | 40020087030 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
