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【資料】Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells
https://junshin.repo.nii.ac.jp/records/598
https://junshin.repo.nii.ac.jp/records/5986785b157-69b5-4775-b7af-3672a7bb2913
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells (7.4 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
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| 公開日 | 2023-04-19 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 【資料】Examining the effect of gemcitabine on DNA double-strand breaks using an immunofluorescence method in mammalian cells | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Radiosensitizationgemcitabine | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | gemcitabine | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 53BP1 | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | DNA double-strand break | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | immunofluorescence | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| 著者 |
森川, 惠子
× 森川, 惠子
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Gemcitabine (dFdC) is a deoxycytidine analogue that is well known for its anti-tumor activity, especially in pancreatic cancer. It is an effective drug for sensitizing cells to radiation, but the exact interaction of dFdC and radiation has not been elucidated. In this study, we evaluated the effect of gemcitabine on radiosensitization in mammalian cells using immunofluorescence (IF) to examine DNA double-strand breaks (DSBs) induced in cell nuclei. To detect the site of DNA DSBs, IF staining was performed to observe phosphorylated p53-binding protein 1 (53BP1)-positive foci over time. Cells were treated with or without 5 μM gemcitabine for 24 hours prior to treatment with or without post-γ-ray irradiation. Gemcitabine could induce DNA DSBs without irradiation, which were not repaired over time. After irradiation, significantly higher numbers of 53BP1 foci were observed in cells pre-treated with gemcitabine than in untreated cells. Higher numbers of 53BP1 foci were confirmed to remain over time in the cells treated with both gemcitabine and irradiation. Overall, our data suggest that the enhanced cell killing effect observed with gemcitabine treatment is dependent on the inhibition of cellular DNA DSB repair mechanisms. | |||||||||||
| 書誌情報 |
純真学園大学雑誌 en : Journal of Junshin Gakuen University 号 13, p. 121-128, 発行日 2023-03-31 |
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| 出版者 | ||||||||||||
| 出版者 | 純真学園大学 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 2186-6481 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA12581584 | |||||||||||
